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Biochemistry

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JoVE Journal Biochemistry
"Cell Surface Capture"-Workflow zur markierungsfreien Quantifizierung des Zelloberflächenproteoms
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"Cell Surface Capture"-Workflow zur markierungsfreien Quantifizierung des Zelloberflächenproteoms

Article DOI: 10.3791/64952-v • 06:31 min • March 24th, 2023
March 24th, 2023 •
1, 1,2, 1,3,4
1Department of Laboratory Medicine, University of California, San Francisco, 2Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, 3Deptartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, 4Chan Zuckerberg Biohub

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Hier beschreiben wir einen Proteomik-Workflow zur Charakterisierung des Zelloberflächenproteoms verschiedener Zelltypen. Dieser Arbeitsablauf umfasst die Anreicherung von Proteinen auf der Zelloberfläche, die anschließende Probenvorbereitung, die Analyse mit einer LC-MS/MS-Plattform und die Datenverarbeitung mit spezieller Software.

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Biochemie Heft 193
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