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Biochemistry

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JoVE Journal Biochemistry
"Cell Surface Capture"-arbeidsflyt for etikettfri kvantifisering av celleoverflateproteomet
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"Cell Surface Capture"-arbeidsflyt for etikettfri kvantifisering av celleoverflateproteomet

Article DOI: 10.3791/64952-v • 06:31 min • March 24th, 2023
March 24th, 2023 •
1, 1,2, 1,3,4
1Department of Laboratory Medicine, University of California, San Francisco, 2Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, 3Deptartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, 4Chan Zuckerberg Biohub

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Her beskriver vi en proteomisk arbeidsflyt for karakterisering av celleoverflateproteomet av forskjellige celletyper. Denne arbeidsflyten inkluderer proteinberikelse på celleoverflaten, påfølgende prøvepreparering, analyse ved hjelp av en LC-MS/MS-plattform og databehandling med spesialisert programvare.

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