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Journal / Biochemistry / "Cell Surface Capture" workflow til etiketfri…
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Biochemistry

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JoVE Journal Biochemistry
"Cell Surface Capture" workflow til etiketfri kvantificering af celleoverfladeproteomet
Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web
Journal (Biochemistry)
Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web
NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode
Journal (Biochemistry)
NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode
 
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"Cell Surface Capture" workflow til etiketfri kvantificering af celleoverfladeproteomet

Article DOI: 10.3791/64952-v • 06:31 min • March 24th, 2023
March 24th, 2023 •
1, 1,2, 1,3,4
1Department of Laboratory Medicine, University of California, San Francisco, 2Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, 3Deptartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, 4Chan Zuckerberg Biohub

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Her beskriver vi en proteomics arbejdsgang til karakterisering af celleoverfladeproteomet af forskellige celletyper. Denne arbejdsgang omfatter celleoverfladeproteinberigelse, efterfølgende prøveforberedelse, analyse ved hjælp af en LC-MS / MS-platform og databehandling med specialiseret software.

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Biokemi nr. 193
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