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Monitoring Stub1-Mediated Pexophagy

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Biology

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JoVE Journal Biology

Monitoring Stub1-Mediated Pexophagy

Monitoring Stub1-Mediated Pexophagy

DOI:

10.3791/65010-v

•

08:26 min

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May 12, 2023

•

Bo-Hua Chen, Wei Yuan Yang²

¹Institute of Biological Chemistry,Academia Sinica, ²Institute of Biochemical Sciences, College of Life Sciences,National Taiwan University

Chapters

00:05Introduction
00:51Preparation of Cells Expressing DiKillerRed or Self‐Labeling Proteins (SLPs) in the Peroxisome Lumen
02:52Staining Peroxisomes with Dye‐Labeled SLP Ligands
03:39ROS‐Stressing of Peroxisomes on a Laser‐Scanning Confocal Microscope
06:25Results: Light‐Activated ROS Production and Stub1‐Mediated Pexophagy
07:50Conclusion

Summary

Automatic Translation

This protocol provides instructions for triggering and monitoring Stub1-mediated pexophagy in live cells.

Tags

Keywords: Peroxisome ROS Organelle Injury Cell Culture Transfection Light-activated ROS HaloTag Confocal Microscopy

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