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Biochemistry

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JoVE Journal Biochemistry
Halvautomatisk fænotypisk analyse af funktionelle 3D-sfæroidcellekulturer
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Halvautomatisk fænotypisk analyse af funktionelle 3D-sfæroidcellekulturer

Article DOI: 10.3791/65086-v • 06:48 min • August 18th, 2023
August 18th, 2023 •
1, 1, 2,3, 2, 3, 1
1Department of Biochemistry, Albert Einstein College of Medicine, 2Department of Biochemistry and Molecular Biology, University of Southern Denmark, 3CelVivo ApS

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Vi præsenterer en protokol til dyrkning af højreproducerbare sfæroider og deres fænotypiske karakterisering ved hjælp af billedoptagelse og proteomics.

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Biokemi udgave 198 Clinostat-inkubator 3D-cellekulturer kræfthepatocytter ikke-kræfthepatocytter leverceller inkubatorer til 3D-celler cellevækstovervågning øjebliksbilleder af cellevækst sfæroidtælling sfærisk måling THLE-3-cellelinje HepG2 / C3A-cellelinje proteomikeksperimenter cellesignalforstyrrelse respiratorisk kædemetabolisme metalafgiftning fænotypisk karakterisering billedoptagelse proteomics-pipeline
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