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JoVE Journal
Immunology and Infection

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富集分枝杆菌的天然和重组细胞外囊泡
 
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富集分枝杆菌的天然和重组细胞外囊泡

Article DOI: 10.3791/65138-v 06:38 min December 8th, 2023
December 8th, 2023

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Summary

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Click here for the English version.

该方案详细介绍了从耻垢分枝杆菌(Msm)的无轴培养物中富集天然分枝杆菌细胞外囊泡(mEV),以及如何设计和富集含有mCherry(红色荧光报告基因)的重组MsmEV。最后,通过富集含有结核分枝杆菌EsxA蛋白的MsmEVs验证了新方法。

Tags

免疫学与感染,第 202 期,细胞外囊泡、重组囊泡、Esat-6、分枝杆菌、ExsA、mCherry、Cfp-29、细菌
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