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用于适应高通量/高含量筛查的细胞内分枝杆菌定量的微观表型检测

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Immunology and Infection

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JoVE Journal Immunology and Infection

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

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用于适应高通量/高含量筛查的细胞内分枝杆菌定量的微观表型检测

DOI:

10.3791/51114-v

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15:28 min

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January 17, 2014

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Christophe. J Queval*¹, Ok-Ryul Song*¹, Vincent Delorme*¹, Raffaella Iantomasi, Romain Veyron-Churlet, Nathalie Deboosère, Valérie Landry, Alain Baulard, Priscille Brodin

¹Inserm U1019 – CNRS UMR 8024, Institut Pasteur de Lille,Université de Lille

Chapters

00:05Title
01:44Preparation of the Bacterial Suspension
03:07High-throughput Genome-wide siRNA Screening
06:16High-throughput Compound Screening
08:27Image Acquisition and Analysis
11:10Results: Mtb Colonization in Host Cells is Inhibited by Silencing Dectin-1 and by Isonazid or Rifampicin Treatment
14:37Conclusion

Summary

Automatic Translation

在这里，我们描述了一种表型检测，适用于小干扰合成RNA（siRNA）、化合物和结核分枝杆菌突变库的高通量/高含量屏幕。该方法依靠使用自动共聚焦显微镜检测荧光标记的荧光结 核分枝杆菌 。

Tags

Keywords: Tuberculosis Mycobacteria Intracellular Phenotypic Assay High-throughput Screening High-content Screening Mycobacterium Tuberculosis Drug Resistance Genetic Screens Chemical Screens Fluorescence Microscopy Image Analysis Host-pathogen Interaction

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