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测量抗体乳鼠心肌细胞对细胞功能的影响
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes
DOI:

13:42 min

March 08, 2013

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Chapters

  • 00:05Title
  • 01:51IgG Isolation from Patient Samples
  • 04:39Isolation of Rat Cardiomyocytes
  • 08:27Fura-2 Staining of Cardiomyocytes
  • 09:43Recording of Cell Shortening and Ca2+ Transients
  • 12:24Results: The Measurement of an IgG Sample Containing Cardiodepressive Antibodies
  • 13:15Conclusion

Summary

Automatic Translation

所提出的方法提供了一种方法扩张型心肌病的患者血浆中的检测功能有效的心性自体抗体,不论特异性抗原,通过分析隔离病人免疫球蛋白对大鼠离体心肌细胞的缩短及细胞内钙瞬变的影响。

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