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Journal
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Immunology and Infection
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测量抗体乳鼠心肌细胞对细胞功能的影响
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Immunology and Infection
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Immunology and Infection
Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes
Please note that all translations are automatically generated.
Click here for the English version.
测量抗体乳鼠心肌细胞对细胞功能的影响
DOI:
10.3791/4237-v
•
13:42 min
•
March 08, 2013
•
Lars G. Eckerle
,
Stephan B. Felix
,
Lars R. Herda
1
Department of Internal Medicine B
,
University Medicine Greifswald
Chapters
00:05
Title
01:51
IgG Isolation from Patient Samples
04:39
Isolation of Rat Cardiomyocytes
08:27
Fura-2 Staining of Cardiomyocytes
09:43
Recording of Cell Shortening and Ca2+ Transients
12:24
Results: The Measurement of an IgG Sample Containing Cardiodepressive Antibodies
13:15
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
所提出的方法提供了一种方法扩张型心肌病的患者血浆中的检测功能有效的心性自体抗体,不论特异性抗原,通过分析隔离病人免疫球蛋白对大鼠离体心肌细胞的缩短及细胞内钙瞬变的影响。
Tags
Measurement
Antibody Effects
Cellular Function
Isolated Cardiomyocytes
Dilated Cardiomyopathy
Heart Failure
Genetic Disposition
Toxic Substances
Autoantibodies
Cardiac Epitopes
Onset And Progression
Disease
Hemodynamic Improvement
Immunoadsorption
Specific Antigens
Immunoassays
Stimulating Autoantibodies
Blocking Autoantibodies
Cardiotropic Antibodies
Functional Regions
Cardiac Proteins
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