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Biology
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Vertical Immobilization Method for Time-Lapse Microscopy Analysis in Filamentous Cyanobacteria
 

Vertical Immobilization Method for Time-Lapse Microscopy Analysis in Filamentous Cyanobacteria

Article DOI: 10.3791/65612-v 07:26 min September 25th, 2023
September 25th, 2023

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Summary

We present a simple and accessible method for filamentous cyanobacterial visualization in the XY plane. A low-melting point agarose matrix was used, allowing the acquisition of images of proteins involved in the division, in a vertical orientation. Therefore, this methodology can be applied to any filamentous organism and different kinds of proteins.

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Keywords: Vertical Immobilization Time-lapse Microscopy Filamentous Cyanobacteria Anabaena Cell Division Z-ring Divisome Component FtsZ-GFP Low-melting-point Agarose Centrifugation Sample Preparation
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