Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Vanessa Cabra; Montserrat Sams&#243;

doi:10.3791/52311

/

Do’s and Don’ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

JoVE Journal
Biology

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JoVE Journal Biology

Do’s and Don’ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

45,900 Views

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09:25 min

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January 09, 2015

DOI:

10.3791/52311-v

DOI:

10.3791/52311-v

•

09:25 min

January 09, 2015

•

45 Views

Vanessa Cabra¹, Montserrat Samsó¹

¹Department of Physiology and Biophysics,Virginia Commonwealth University

Summary

This paper describes the cryo-electron microscopy methodology, which is used to obtain high quality microscopic images of macromolecules in their near-native state. The method yields images suitable for further computerized processing using the single particle approach, devised to generate the 3D reconstruction of a macromolecule.