Modeling Multiple Sclerosis in the Two Sexes: MOG<sub>35-55</sub>-Induced Experimental Autoimmune Encephalomyelitis

Brigitta Bonaldo; Antonino Casile; Francesca Montarolo; Antonio Bertolotto

doi:10.3791/65778

Journal

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Neuroscience

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Modeling Multiple Sclerosis in the Two Sexes: MOG_35-55-Induced Experimental Autoimmune Encephalomyelitis

Modeling Multiple Sclerosis in the Two Sexes: MOG<sub>35-55</sub>-Induced Experimental Autoimmune Encephalomyelitis

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Modeling Multiple Sclerosis in the Two Sexes: MOG_35-55-Induced Experimental Autoimmune Encephalomyelitis

1,330 Views

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05:44 min

•

October 13, 2023

DOI:

10.3791/65778-v

DOI:

10.3791/65778-v

•

05:44 min

October 13, 2023

•

1297 Views

Brigitta Bonaldo^1,2, Antonino Casile^1,2,3, Francesca Montarolo^1,2, Antonio Bertolotto^1,4

¹Neuroscience Institute Cavalieri Ottolenghi (NICO)²Department of Neuroscience “Rita Levi-Montalcini”,University of Turin ³School of Pharmacy, Pharmacology Unit,University of Camerino ⁴Koelliker Hospital

Summary

Experimental autoimmune encephalomyelitis is one of the most widely used murine models of multiple sclerosis. In the current protocol, C57BL/6J mice of both sexes are immunized with myelin oligodendrocyte glycoprotein peptide, resulting mainly in ascending paresis of the tail and limbs. Here we discuss the protocol of EAE induction and evaluation.