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Journal / Genetics / CRISPR-Cas9 genomredigering af rotteembryoner ved …
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Genetics
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JoVE Journal Genetics
CRISPR-Cas9 genomredigering af rotteembryoner ved hjælp af adenoassocieret virus (AAV) og 2-celle embryoelektroporation
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CRISPR-Cas9 genomredigering af rotteembryoner ved hjælp af adenoassocieret virus (AAV) og 2-celle embryoelektroporation

Article DOI: 10.3791/66069-v • 08:24 min • March 15th, 2024
March 15th, 2024 •
1,2,4, 2, 3, 3, 3, 1,2,3,4
1Animal Modeling Core, University of Missouri, 2Comparative Medicine Program, University of Missouri, 3Rat Resource and Research Center, University of Missouri, 4Department of Veterinary Pathobiology, University of Missouri

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Denne protokol præsenterer en optimeret tilgang til fremstilling af genetisk modificerede rottemodeller. Adeno-associeret virus (AAV) bruges til at levere en DNA-reparationsskabelon, og elektroporation bruges til at levere CRISPR-Cas9-reagenser for at fuldføre genomredigeringsprocessen i 2-celleembryo.

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Denne måned i JoVE udgave 205 Adeno-associeret virus (AAV) genomredigering CRISPR-Cas elektroporation 2-cellede embryoner knock-in rottedyremodel
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