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Dissection of Larval CNS in Drosophila Melanogaster
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Dissection of Larval CNS in Drosophila Melanogaster

Dissection of Larval CNS in Drosophila Melanogaster

DOI:
10.3791/85-v

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06:29 min

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November 09, 2006

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,
1Department of Molecular Biology,Princeton University

Chapters

  • 00:00Introduction
  • 00:08Isolating larvae
  • 01:22Dissection
  • 04:00Example: gene expression in Drosophila CNS
  • 04:13Discussion

Summary

Automatic Translation

Automatic Translation

In this article we demonstrate how to dissect the central nervous system from third instar Drosophila larvae.

Tags

DissectionLarval CNSDrosophila MelanogasterImmunohistochemistryExpressionNovel ProteinsMarker ProteinsStainingWhole LarvaeTough CuticleAntibodiesBody CavityFixingTearingFine ForcepsInside-outExposureFixationStaining StepsMounting SamplesGlass SlidesRepresentative ImagesEve Transcription FactorNeuronsPractical UsesPotential Difficulties

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