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Biology
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从P0新生大鼠海马神经元的原代培养
JoVE Journal
Biology
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JoVE Journal
Biology
Primary Culture of Hippocampal Neurons from P0 Newborn Rats
Please note that all translations are automatically generated.
Click here for the English version.
从P0新生大鼠海马神经元的原代培养
DOI:
10.3791/895-v
•
07:18 min
•
September 29, 2008
•
Joseph Nunez
1
Department of Psychology
,
Michigan State University (MSU)
Chapters
00:00
Title
00:15
Introduction
01:04
Preparing Tools and Reagents
02:40
Isolating the Hippocampi
04:21
Dissociation of Hippocampi
06:09
Imaging Hippocampal Neurons
07:02
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
解剖和个人的大脑区域的细胞的生长,促进细胞和生理参数的调查。我们描述了一个初级细胞培养的方法,在无血清环境产生的神经元丰富的文化。
Tags
Hippocampal Neurons
P0 Newborn Rats
Primary Culture
Physiological Properties
Involvement
Learning And Memory
Neuroscientists
Individual Cell
Single Synapse Level
Isolate
Grow
Cultures
Two Weeks
Ratiometric Calcium Imaging
Protocol
Brain Regions
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