JoVE Journal
Immunology and Infection
Immunology and Infection
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Chapters
Summary
Please note that all translations are automatically generated.
This article describes a method to obtain a three-dimensional (3D) structure of helically assembled molecules using cryo-electron microscopy. In this protocol, we use HIV-1 capsid assemblies to illustrate the detailed 3D reconstruction procedure for achieving a density map by the iterative helical real-space reconstruction method.