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Sampling Human Indigenous Saliva Peptidome Using a Lollipop-Like Ultrafiltration Probe: Simplify and Enhance Peptide Detection for Clinical Mass Spectrometry
JoVE Journal
Medicine
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JoVE Journal Medicine
Sampling Human Indigenous Saliva Peptidome Using a Lollipop-Like Ultrafiltration Probe: Simplify and Enhance Peptide Detection for Clinical Mass Spectrometry
DOI:

08:37 min

August 07, 2012

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Chapters

  • 00:05Title
  • 01:43Creation of LLUF Probes
  • 02:23Saliva Collection
  • 03:14Nano-LC-LTQ MS Acquisition and Analysis
  • 04:47Removal of Oral Bacteria with LLUF Probes
  • 05:22Results: Identification of an Indigenous Saliva Peptidome
  • 07:34Conclusion

Summary

Automatic Translation

Considering saliva sampling for future clinical application, a lollipop-like ultrafiltration (LLUF) probe was fabricated to fit in the human oral cavity. Direct analysis of undigested saliva by NanoLC-LTQ mass spectrometry demonstrated the ability of LLUF probes to remove large proteins and high abundance proteins, and make low-abundant peptides more detectable.

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