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Measurement of Vacuolar and Cytosolic pH In Vivo in Yeast Cell Suspensions
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Measurement of Vacuolar and Cytosolic pH In Vivo in Yeast Cell Suspensions

Measurement of Vacuolar and Cytosolic pH In Vivo in Yeast Cell Suspensions

DOI:
10.3791/50261-v

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13:55 min

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April 19, 2013

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, ,
1Department of Biochemistry and Molecular Biology,SUNY Upstate Medical University

Chapters

  • 00:05Title
  • 02:12Measurement of Vacuolar pH In Vivo using BCECF-AM
  • 07:17Measurement of Cytosolic pH In Vivo using Yeast pHluorin
  • 11:54Results: Vacuolar and Cytosolic pH Changes in Response to Glucose and Other Stimuli
  • 13:25Conclusion

Summary

Automatic Translation

Automatic Translation

Vacuolar and cytosolic pH can be measured in live yeast (S. cerevisiae) cells using ratiometric fluorescent dyes localized to specific cellular compartments. We describe procedures for measuring vacuolar pH with BCECF-AM, which localizes to the vacuole in yeast, and cytosolic pH with a cytosolic ratiometric pH-sensitive GFP (yeast pHluorin).

Tags

Vacuolar PHCytosolic PHPH HomeostasisYeastBCECFYeast PHluorinRatiometric MeasurementFluorescenceFluorimeterFluorescence Plate Reader

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