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A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells

A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells

DOI:
10.3791/50606-v

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16:04 min

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June 19, 2013

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, , ,
1Mork Family Department of Chemical Engineering and Materials Science, Viterbi School of Engineering,University of Southern California, Los Angeles

Chapters

  • 00:05Title
  • 01:59Generating the GPRS Packaging Cell Line: A Tet-dependent SVGmu Cell Line
  • 05:33Constructing DC-LV Producer Cells by Concatemer Array Transfection
  • 09:14Evaluating Viral Production of Each Cell Clone
  • 10:54Producing and Concentrating Lentiviral Vectors
  • 12:16Results: Isolation of High-titer Lentiviral Vectors that Specifically Target Dendritic Cells
  • 15:15Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we use retroviral transduction and concatemeric transfection to create a cell line that can express the components of a lentiviral vector (LV) in the absence of tetracycline. This LV encodes GFP and is pseudotyped with a glycoprotein, SVGmu, which is specific for a receptor on dendritic cells.

Tags

Lentiviral VectorsHigh-titerDendritic CellsTet-offProducer Cell LineConcatemeric DNA TransfectionReporter GeneTransductionT Cell Immune Response

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