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Journal / Immunology and Infection / En Microplate Assay å vurdere Kjemiske Effekter…
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Immunology and Infection

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JoVE Journal Immunology and Infection
En Microplate Assay å vurdere Kjemiske Effekter på RBL-2H3 Mast Cell degranulation
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En Microplate Assay å vurdere Kjemiske Effekter på RBL-2H3 Mast Cell degranulation: Effekter av Triklosan uten bruk av et organisk løsemiddel

Article DOI: 10.3791/50671-v • 17:35 min • November 1st, 2013
November 1st, 2013 •
*1,2, *1,2, 1, 1,2
1Department of Molecular and Biomedical Sciences, University of Maine, Orono, 2Graduate School of Biomedical Sciences and Engineering, University of Maine, Orono
* These authors contributed equally

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Mastcelle degranulering, frigjøring av allergiske mediatorer, er viktig ved allergi, astma, og parasitt forsvar. Her kan vi demonstrere teknikker en for å vurdere effekter av legemidler og giftstoffer på degranulation, metodikk nylig brukt til å stille den kraftige hemmende effekten av antibakterielt middel triklosan to.

Tags

Immunologi mast celle basofilrelatert degranulation RBL-2H3 triklosan irgasan antibakteriell β-hexosaminidase allergi astma miljøgifter ionophore antigen fluorescens microplate UV-Vis
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