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Journal
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Immunology and Infection
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微孔板的检测,以评估RBL-2H3肥大细胞脱颗粒:三氯生的影响不使用有机溶剂的化学效应
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Immunology and Infection
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JoVE Journal
Immunology and Infection
A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent
Please note that all translations are automatically generated.
Click here for the English version.
微孔板的检测,以评估RBL-2H3肥大细胞脱颗粒:三氯生的影响不使用有机溶剂的化学效应
DOI:
10.3791/50671-v
•
17:35 min
•
November 01, 2013
•
Lisa M. Weatherly*
1,2
,
Rachel H. Kennedy*
1,2
,
Juyoung Shim
,
Julie A. Gosse
2
1
Department of Molecular and Biomedical Sciences
,
University of Maine, Orono
,
2
Graduate School of Biomedical Sciences and Engineering
,
University of Maine, Orono
Chapters
00:05
Title
02:45
Preparation of Cells
04:06
Preparation of Triclosan (TCS)
06:12
Antigen-stimulated Degranulation Assay Using RBL-2H3 Cells
12:13
Results: TCS Causes a Significant Dose-responsive Inhibition of Mas Cell Degranulation
16:38
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
肥大细胞脱颗粒,释放过敏介质,过敏,哮喘和寄生虫防御是很重要的。在这里,我们展示了对脱颗粒的药物和毒物的影响评估的
技术
,方法,最近利用抗菌剂三氯生
2,
表现出强大的抑制作用。
Tags
Microplate Assay
RBL-2H3 Mast Cells
Mast Cell Degranulation
Triclosan
Allergic Mediators
β-hexosaminidase
High-throughput Screening
Organic Solvent-free
UV-Vis Spectrophotometry
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