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Immunology and Infection

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JoVE Journal Immunology and Infection
Identifiering av nya gener associerade med alginat Produktion i
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Identifiering av nya gener associerade med alginat Produktion i

Article DOI: 10.3791/51346-v • 08:57 min • March 10th, 2014
March 10th, 2014 •
1, 1, 1
1Department of Biochemistry and Microbiology, Joan C. Edwards School of Medicine, Marshall University

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Här beskriver vi ett protokoll med hjälp av mini-himar1 mariner transposon mutagenes för att generera en hög densitet insättning mutant bibliotek till skärmen, isolera och identifiera nya alginat tillsynsmyndigheter i prototypiska Pseudomonas aeruginosa stam PAO1.

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Immunology , Alginat mucoidy mutagenes mini- pFAC
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