Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content.

Generation af murine pacemaker celleaggregater Baseret på ES-celle-programmering i kombination med Myh6-Promotor-Selection
 
Click here for the English version

Generation af murine pacemaker celleaggregater Baseret på ES-celle-programmering i kombination med Myh6-Promotor-Selection

Article DOI: 10.3791/52465-v 08:52 min February 17th, 2015
February 17th, 2015

Chapters

Summary

Please note that all translations are automatically generated.

Click here for the English version.

Denne protokol beskriver, hvordan man producere funktionel sinus nodal væv fra murine pluripotente stamceller (PSC). T-BOX3 (TBX3) overekspression plus kardial myosin-tung-kæde (Myh6) promoter antibiotisk selektion fører til meget rene pacemaker celleaggregater. Disse "induceret sinoatriale-organer" ("iSABs") indeholder over 80% pacemaker celler, viser stærkt forøgede bankende satser og er i stand til at pace myocardium ex vivo.

Tags

Developmental Biology murine pluripotente stamceller (PSC) syg sinus-syndrom iSABs inducerede sinoatrialt organer kardiomyocytter pacemaker
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter