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Implementering Patch Clamp og Live Fluorescensmikroskopi til Monitor funksjonelle egenskaper nyisolerte PKD Epitel
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Implementering Patch Clamp og Live Fluorescensmikroskopi til Monitor funksjonelle egenskaper nyisolerte PKD Epitel

Article DOI: 10.3791/53035-v • 08:46 min • September 1st, 2015
September 1st, 2015 •
1, 1, 1, 1, 2, 1
1Department of Physiology, Medical College of Wisconsin, 2Department of Integrative Biology & Pharmacology, University of Texas Health Science Center at Houston

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Ionekanaler uttrykt i nyretubuli epitel spille en betydelig rolle i patologi polycystisk nyresykdom. Her beskriver vi eksperimentelle protokollene som brukes til å utføre patch-clamp analyse og intracellulær kalsium nivå målinger ved cystisk epitel fersk isolert fra gnager nyrer.

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Medisin Patch-klemme polycystisk nyresykdom ARPKD ADPKD nyre intracellulær kalsium Fura-2 AM nephron cyste utvikling polycystin
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