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实施膜片钳和活荧光显微镜监视新鲜分离的PKD上皮细胞的功能特性
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Biology
Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium
Please note that all translations are automatically generated.
Click here for the English version.
实施膜片钳和活荧光显微镜监视新鲜分离的PKD上皮细胞的功能特性
DOI:
10.3791/53035-v
•
08:46 min
•
September 01, 2015
•
Tengis S. Pavlov
,
Daria V. Ilatovskaya
,
Oleg Palygin
,
Vladislav Levchenko
,
Oleh Pochynyuk
,
Alexander Staruschenko
1
Department of Physiology
,
Medical College of Wisconsin
,
2
Department of Integrative Biology & Pharmacology
,
University of Texas Health Science Center at Houston
Chapters
00:05
Title
02:14
Isolation of Renal Cysts and Connecting Tubules/Collecting Duct Segments
05:02
Single Channel Patch-clamp Electrophysiology
05:32
Ratiometric Epifluorescence Measurement of Intracellular Calcium Concentration in the Epithelial Cells
06:55
Results: Effect of Benzamil Treatment and ATP in the Cystic Cells of PCK Rats
08:08
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
表现在肾小管上皮细胞离子通道在多囊肾病的病理一个显著的作用。这里,我们描述用于执行囊性上皮从啮齿动物肾脏新鲜分离的膜片钳分析和细胞内钙水平的测量的实验方案。
Tags
Patch Clamp
Live Fluorescence Microscopy
Polycystic Kidney Disease
PKD
ARPKD
Ion Channels
Calcium Signaling
Epithelial Monolayer
Ex Vivo Analysis
PCK Rat
Sprague Dawley Rat
Native Setting
Epithelial Isolation
Immunostaining
Primary Culture
Biochemical Assays
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