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JoVE Journal
Biology

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实施膜片钳和活荧光显微镜监视新鲜分离的PKD上皮细胞的功能特性
 
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实施膜片钳和活荧光显微镜监视新鲜分离的PKD上皮细胞的功能特性

Article DOI: 10.3791/53035
September 1st, 2015

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Summary September 1st, 2015

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表现在肾小管上皮细胞离子通道在多囊肾病的病理一个显著的作用。这里,我们描述用于执行囊性上皮从啮齿动物肾脏新鲜分离的膜片钳分析和细胞内钙水平的测量的实验方案。

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