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JoVE Journal Bioengineering
Udarbejdelse af 3D Kollagen Gels og mikrokanalerne for Studiet af 3D Interactions
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Udarbejdelse af 3D Kollagen Gels og mikrokanalerne for Studiet af 3D Interactions

Article DOI: 10.3791/53989-v • 10:24 min • May 9th, 2016
May 9th, 2016 •
1,2, 1, 1, 1, 2,3,4, 1,2,5
1Department of Cell and Regenerative Biology, University of Wisconsin-Madison, 2Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison, 3Department of Biomedical Engineering, University of Wisconsin-Madison, 4Morgridge Institute for Research, University of Wisconsin-Madison, 5Paul P. Carbone Comprehensive Cancer center, University of Wisconsin-Madison

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Kollagen er et centralt element i ECM, og giver vigtige signaler til flere cellulære processer lige fra migration til differentiering og proliferation. Forudsat her er en protokol til indlejring celler i 3D-collagen hydrogeler, og en mere avanceret teknik til at generere randomiserede eller justeret collagen matricer hjælp PDMS mikrokanaler.

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Bioengineering Collagen mikrofluidkanaler 3D matricer ECM kollagen tilpasning tumor mikromiljø
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