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JoVE Journal Bioengineering
Préparation de la 3D collagène Gels et microcanaux pour l'étude des interactions 3D
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Préparation de la 3D collagène Gels et microcanaux pour l'étude des interactions 3D

Article DOI: 10.3791/53989-v • 10:24 min • May 9th, 2016
May 9th, 2016 •
1,2, 1, 1, 1, 2,3,4, 1,2,5
1Department of Cell and Regenerative Biology, University of Wisconsin-Madison, 2Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison, 3Department of Biomedical Engineering, University of Wisconsin-Madison, 4Morgridge Institute for Research, University of Wisconsin-Madison, 5Paul P. Carbone Comprehensive Cancer center, University of Wisconsin-Madison

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Le collagène est une composante essentielle de l'ECM, et fournit des indices essentiels pour plusieurs processus cellulaires allant de la migration vers la différenciation et la prolifération. Pourvu ici est un protocole pour l'intégration des cellules dans les hydrogels de collagène 3D, et une technique plus avancée pour générer des matrices de collagène randomisées ou alignées à l'aide de microcanaux PDMS.

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Bioengineering numéro 111 Collagène canaux microfluidiques matrices 3D ECM l'alignement de collagène microenvironnement de la tumeur
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