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Novel Approach for Simultaneous Recording of Renal Sympathetic Nerve Activity and Blood Pressure with Intravenous Infusion in Conscious, Unrestrained Mice.
JoVE Journal
Medicine
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JoVE Journal Medicine
Novel Approach for Simultaneous Recording of Renal Sympathetic Nerve Activity and Blood Pressure with Intravenous Infusion in Conscious, Unrestrained Mice.
DOI:

11:08 min

February 14, 2018

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Chapters

  • 00:04Title
  • 00:44Surgical Implantation of the RSNA Electrode
  • 03:58Implantation of Blood Pressure Radiotelemeter
  • 06:02Implantation and Exteriorization of the Jugular Venous Catheter
  • 07:45Sample Experimental Protocol and Validation of RSNA Signal
  • 08:40Results: Renal Sympathetic Nerve Activity (RSNA) in Conscious Mice
  • 10:10Conclusion

Summary

Automatic Translation

Anesthetized mice exhibit non-physiological systemic blood pressure, which precludes meaningful assessment of autonomic tone given the intimate relationship between blood pressure and the autonomic nervous system. Thus, a novel method to simultaneously record renal sympathetic nerve activity and blood pressure with intravenous infusion in conscious mice is outlined.

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