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Biochemistry

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JoVE Journal Biochemistry
Single-molekyle Super-resolution Imaging af Phosphatidylinositol 4,5-bisphosphat i plasmamembranen med Novel fluorescerende prober
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Single-molekyle Super-resolution Imaging af Phosphatidylinositol 4,5-bisphosphat i plasmamembranen med Novel fluorescerende prober

Article DOI: 10.3791/54466-v • 07:26 min • October 15th, 2016
October 15th, 2016 •
1, 1
1Department of Neuroscience, University of Wisconsin-Madison

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PI (4,5) P2 regulerer forskellige cellulære funktioner, men dens nanoskala organisation i cellen plasmamembranen er dårligt forstået. Ved mærkning PI (4,5) P2 med en tofarvet fluorescerende probe fusioneret til pleckstrin homologidomænet beskriver vi en ny tilgang til at studere PI (4,5) P2 rumlige fordeling i plasmamembranen på nanometerskala .

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Biokemi Super-opløsning billeddannelse PALM Phosphatidylinositol 4,5-bisphosphat (PI (4,5) P2) cellemembran ark phosphoinositid betaceller
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