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Journal / Developmental Biology / Forberedelse og Kultur Myogenic precursorceller / …
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Forberedelse og Kultur Myogenic precursorceller / Primær Myoblaster af skeletmuskulatur af Voksen og Aged Mennesker
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Forberedelse og Kultur Myogenic precursorceller / Primær Myoblaster af skeletmuskulatur af Voksen og Aged Mennesker

Article DOI: 10.3791/55047-v • 10:10 min • February 16th, 2017
February 16th, 2017 •
1, 1, 1,2, 1
1Institute of Ageing and Chronic Disease, University of Liverpool, 2Aintree University Hospital

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Denne protokol beskriver en robust, reproducerbar og enkel fremgangsmåde til isolering og dyrkning af myoblast progenitorceller fra skeletmuskel af voksne og ældre mennesker. De muskler, der anvendes her, omfatter fod og ben muskler. Denne tilgang muliggør isolering af en beriget population af primære myoblaster for funktionelle studier.

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Developmental Biology primære myoblaster muskel aldring regenerering microRNA menneske
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