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Journal / Neuroscience / Rekombinante α-β und γ-Synucleins stimulieren…
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Neuroscience
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JoVE Journal Neuroscience
Rekombinante α-β und γ-Synucleins stimulieren Protein Phosphatase 2A katalytische Untereinheit Aktivität in Zelle frei Assays
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Rekombinante α-β und γ-Synucleins stimulieren Protein Phosphatase 2A katalytische Untereinheit Aktivität in Zelle frei Assays

Article DOI: 10.3791/55361-v • 09:36 min • August 13th, 2017
August 13th, 2017 •
1, 1, 1, 1, 1, 1
1Department of Biomedical Sciences, Center of Emphasis in Neurosciences, Graduate School of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center El Paso

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Diese Veröffentlichung enthält ein Protokoll zeigt, wie die Aktivität des rekombinanten Proteins Phosphatase 2A katalytische Untereinheit (PP2Ac) Messen in Reaktion auf rekombinante α-Synuclein, β-Synuclein oder γ-Synuclein Proteine mit einem einfachen farbmetrischen Assay. Feststellungen zur Spezifität der α-Synuclein in Richtung PP2Ac zeigen wir im Gehirn der Maus.

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Neurowissenschaften Ausgabe 126 farbmetrische Assay Dephosphorylation Malachit Phosphat Phosphatase-Aktivität PP2A katalytische Untereinheit rekombinante Proteine Synucleins Threonin phosphopeptide
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