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Immunology and Infection

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JoVE Journal Immunology and Infection
Precision-cut Mouse Lung skivor att visualisera Live Pulmonary dendritiska celler
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Precision-cut Mouse Lung skivor att visualisera Live Pulmonary dendritiska celler

Article DOI: 10.3791/55465-v • 09:33 min • April 5th, 2017
April 5th, 2017 •
1, 1, 1, 1
1Immunity, Inflammation, and Disease Laboratory, Division of Intramural Research, National Institute of Environmental Health Sciences, NIH

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Vi beskriver ett förfarande för generering Precision-cut Lung skivor (PCLS) och immunfärgning dem att visualisera lokalisering av olika immuncelltyper i lungan. Vår protokoll kan utvidgas till att visualisera placeringen och funktionen hos många olika celltyper under olika förhållanden.

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Immunology levande cell imaging precisionsslipning lungsektioner lunga dendritiska celler lokalisering konfokalmikroskopi
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