JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
精密切割的小鼠肺切片可视化现场肺树突状细胞
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells
Please note that all translations are automatically generated.
Click here for the English version.
精密切割的小鼠肺切片可视化现场肺树突状细胞
DOI:
10.3791/55465-v
•
09:33 min
•
April 05, 2017
•
Miranda R. Lyons-Cohen
,
Seddon Y. Thomas
,
Donald N. Cook
,
Hideki Nakano
1
Immunity, Inflammation, and Disease Laboratory, Division of Intramural Research
,
National Institute of Environmental Health Sciences, NIH
Chapters
00:05
Title
00:51
Lung Harvest and Preparation
02:36
Lung Slicing
04:29
Static PCLS Imaging
05:44
PCLS Live-cell Imaging
07:01
Results: Representative Immune Cell Population Identification
08:36
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
我们描述了用于产生精密切割肺片(PCLS)和免疫它们以显现各种免疫细胞类型的定位在肺中的方法。我们的协议可以扩展在各种条件下可视化的位置和许多不同细胞类型的功能。
Tags
Precision-cut Lung Slices
PCLS
Immune Cell Trafficking
Lung Immunology
Airway Contraction
Mouse Lung
Dendritic Cells
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
小鼠肺树突状细胞的分离
过敏性气道炎症在肺树突状细胞的成熟和迁移分析
从老鼠肠道树突状细胞和巨噬细胞的分离和表征
右心室收缩压测量组合在小鼠的肺和免疫组织样品的收获
绿脓杆菌
引起的肺损伤模型
在鸡胚的流感病毒传播
嗜肺军团菌
外膜囊泡:隔离及对巨噬细胞促炎潜力分析
人肺树突状细胞:空间分布与表型鉴定支气管活检采用免疫组化和流式细胞仪
人精切肺切片中物质的细胞毒性及免疫调节作用的评价
人干扰素α超灵敏单分子阵列数字酶联免疫吸附试验的研制与验证
Read Article