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Journal / Neuroscience / Motor Nöron benzeri NSC-34 Hücrelerinde Gerçek…
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JoVE Journal Neuroscience
Motor Nöron benzeri NSC-34 Hücrelerinde Gerçek Zamanlı Nükleositoplazmik Taşınımı Ölçüm Testi
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Motor Nöron benzeri NSC-34 Hücrelerinde Gerçek Zamanlı Nükleositoplazmik Taşınımı Ölçüm Testi

Article DOI: 10.3791/55676-v • 08:53 min • May 16th, 2017
May 16th, 2017 •
1, 1, 1,2
1Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, 2The Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev

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Bu protokol, bir NLS-NES-GFP proteininin nükleer ithalatında gerçek zamanlı değişikliği niceleyerek motor nöron benzeri NSC-34 hücrelerindeki nükleositoplazmik taşınım oranını duyarlı bir şekilde ölçmek için bir yöntem açıklamaktadır.

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Nörobilim Sayı 123 nükleositoplazmik nakil mekik GFP NSC-34 hücreleri C9orf72 ALS
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