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测定在运动神经元样NSC-34细胞中实时测量核细胞质运输
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JoVE Journal Neuroscience
Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells
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测定在运动神经元样NSC-34细胞中实时测量核细胞质运输

DOI:
10.3791/55676-v

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08:53 min

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May 16, 2017

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, ,
1Department of Physiology and Cell Biology, Faculty of Health Sciences,Ben-Gurion University of the Negev, 2The Zlotowski Center for Neuroscience,Ben-Gurion University of the Negev

Chapters

  • 00:05Title
  • 00:48Cell Line Preparation
  • 02:31Cell Transfection and Microscopic Analysis
  • 04:54Protein Expression Validation
  • 05:58Results: Representative Shutle-GFP Expression Analyses
  • 08:01Conclusion

Summary

Automatic Translation

Automatic Translation

该方案描述了一种通过量化NLS-NES-GFP蛋白的核进口的实时变化来敏感地测量运动神经元样NSC-34细胞内的核胞质转运速率的方法。

Tags

Nucleocytoplasmic TransportReal-time AssayMotor Neuron-like NSC-34 CellsALSCell CultureCell TransfectionShuttle-GFPNuclear Marker

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