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JoVE Journal Developmental Biology
Omprogrammering primære fostervand og membran celler til Pluripotency i Xeno-fri betingelser
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Omprogrammering primære fostervand og membran celler til Pluripotency i Xeno-fri betingelser

Article DOI: 10.3791/56003-v • 09:34 min • November 27th, 2017
November 27th, 2017 •
1, 2, 1, 3, 3,4,5, 1, 1
1Mitchell Cancer Institute, University of South Alabama, 2College of Medicine, University of South Alabama, 3Institute for Regenerative Medicine, University of Zurich, 4Department of Dermatology, University Hospital Zurich, 5Center for Applied Biotechnology and Molecular Medicine (CABMM), University of Zurich - Irchel Campus

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Denne protokol beskriver omprogrammering af primære fostervand væske og membran mesenkymale stamceller til induceret pluripotente stamceller ved hjælp af en ikke-integrere episomal tilgang i fuldt kemisk definerede betingelser. Procedurer for udvinding, kultur, omprogrammering og karakterisering af de resulterende inducerede pluripotente stamceller af strenge metoder er beskrevet.

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Udviklingsmæssige biologi sag 129 iPSC fostervand dyr omprogrammering episomal E8 medium vitronectin
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