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Cancer Research

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JoVE Journal Cancer Research
Et-trins protokol til evaluering af tilstanden af stråling-induceret Clonogenic celledød af Fluorescens mikroskopi
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Et-trins protokol til evaluering af tilstanden af stråling-induceret Clonogenic celledød af Fluorescens mikroskopi

Article DOI: 10.3791/56338-v • 06:47 min • October 23rd, 2017
October 23rd, 2017 •
1, 2, 1, 1
1Department of Radiation Oncology, Gunma University Graduate School of Medicine, 2Advanced Scientific Research Leaders Development Unit, Gunma University Graduate School of Medicine

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Forskning på ioniserende stråling (IR)-induceret clonogenic celledød er vigtigt for at forstå virkningerne af IR på Maligne tumorer og normale væv. Her, vi beskriver en et-trins analyse til vurdering af de vigtigste former for IR-induceret clonogenic celledød baseret på morfologi af 4', 6-diamidino-2-phenylindole dihydrochlorid (DAPI)-farves kerner visualiseret ved Fluorescens mikroskopi.

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Kræftforskning spørgsmålet 128 stråling biologi ioniserende stråling clonogenic celledød apoptose mitotiske katastrofe cellulære gulne Fluorescens mikroskopi DAPI farvning
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