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JoVE Journal Cancer Research
FluorescensMikroskopi til ATP Internalization medieret af makropinocytose i menneskelige tumorceller og tumor-xenografted mus
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FluorescensMikroskopi til ATP Internalization medieret af makropinocytose i menneskelige tumorceller og tumor-xenografted mus

Article DOI: 10.3791/62768-v • 08:31 min • June 30th, 2021
June 30th, 2021 •
*1,2,3, *4, 1,5, 4, 1,2,4, 1,2,4, 6, 1,2,4,7
1Department of Biological Sciences, Ohio University, 2Molecular and Cellular Biology Program, Ohio University, 3Neuroscience Program, Ohio University, 4Edison Biotechnology Institute, Ohio University, 5Translational Biomedical Sciences Program, Ohio University, 6Honors Tutorial College, Ohio University, 7Department of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University
* These authors contributed equally

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Vi udviklede en reproducerbar metode til at visualisere internaliseringen af ikkehydrolyzable fluorescerende adenosin triphosphat (ATP), en ATP surrogat, med høj cellulær opløsning. Vi validerede vores metode ved hjælp af uafhængige in vitro og in vivo assays-human tumor celle linjer og immundefekt mus xenografted med human tumor væv.

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