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Journal / Genetics / Poolet shRNA skærmen for reaktivering af MeCP2 på …
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JoVE Journal Genetics
Poolet shRNA skærmen for reaktivering af MeCP2 på det inaktive X kromosom
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Poolet shRNA skærmen for reaktivering af MeCP2 på det inaktive X kromosom

Article DOI: 10.3791/56398-v • 11:15 min • March 2nd, 2018
March 2nd, 2018 •
1,2, 1, 1, 1, 1, 1, 1, 3, 1,4
1Clinical Research Division, Fred Hutchinson Cancer Research Center, 2Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 3Epigenetics Program, Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, 4Departments of Medicine and Biochemistry, University of Washington

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Vi rapporterer en lille hårnål RNA (shRNA) og næste generation sequencing-baseret protokol til at identificere regulatorer af x-kromosom Inaktiveringen i en murine cellelinie med firefly luciferase og hygromycin resistensgener smeltet til methyl CpG bindende protein 2) MeCP2) gen på det inaktive X kromosom.

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Genetik sag 133 x-kromosom Inaktiveringen Epigenetik MeCP2 Rett syndrom XIST shRNA screening
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