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Analisi delle cellule immuni in singolo nervo sciatico e del ganglio della radice dorsale da un singolo Mouse mediante citometria a flusso
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Analisi delle cellule immuni in singolo nervo sciatico e del ganglio della radice dorsale da un singolo Mouse mediante citometria a flusso

Article DOI: 10.3791/56538-v • 12:34 min • December 6th, 2017
December 6th, 2017 •
1, 1,2,3, 1,3
1Department of Medicine I and Clinical Chemistry, University Hospital of Heidelberg, 2Institute for Diabetes and Cancer IDC Helmholtz Center Munich, Germany & Joint Heidelberg-IDC Translational Diabetes Program, 3German Center for Diabetes Research (DZD)

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Analisi quantitativa del contenuto della cella all'interno del nervo sciatico murino è difficile a causa della scarsità del tessuto. Questo protocollo descrive un metodo per la digestione del tessuto e preparazione che fornisce cellule sufficienti per analisi di citometria a flusso delle popolazioni delle cellule immuni dai nervi dei topi individuali.

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Neuroscienze problema 130 citometria a flusso mouse nervo sciatico macrofago infiammazione leucociti neuropatia
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