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Bioengineering

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JoVE Journal Bioengineering
Visuel genkendelse af flere nukleinsyrer i en kapillær Array
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Visuel genkendelse af flere nukleinsyrer i en kapillær Array

Article DOI: 10.3791/56597-v • 08:56 min • November 15th, 2017
November 15th, 2017 •
*1,2,3, *1,2,3, *4, 4, 1,2,3, 4,5, 1,2,3, 6, 6, 4, 1,2,3
1Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, 2State Key Laboratory of Oncogenes and Related Genes, 3School of Biomedical Engineering, Shanghai Jiao Tong University, 4Collaborative Innovation Center for Biosafety of GMOs, National Center for the Molecular Characterization of Genetically Modified Organisms, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 5Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, 6Department of Biomedical Engineering, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine, Tsinghua University
* These authors contributed equally

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Denne protokol beskriver fabrikation af en lille, klar-til-brug kassette, der kan anvendes til visuel genkendelse af flere nukleinsyrer i en enkelt, test, der er nem at betjene. I denne tilgang, blev en kapillær array brugt til multiplex og yderst effektiv påvisning af GMO mål.

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Bioteknologi sag 129 kapillær array lampe multiplex nukleinsyre påvisning modificerede genetisk organismer (GMO) nem at betjene visuel opdagelse
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