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Påvisning af Endotoxin i Nano-formuleringer bruger Limulus Amoebocyte Lysate (LAL) Assays
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Bioengineering
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JoVE Journal Bioengineering
Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays

Påvisning af Endotoxin i Nano-formuleringer bruger Limulus Amoebocyte Lysate (LAL) Assays

DOI:
10.3791/58830-v

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06:15 min

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January 30, 2019

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1Nanotechnology Characterization Lab., Cancer Research Technology Program,Frederick National Laboratory for Cancer Research sponsored by National Cancer Institute

Chapters

  • 00:04Title
  • 00:32Turbidity Limulus Amoebocyte Lysate (LAL) Assay
  • 02:26Sample Loading and Preparation and Gel-Clot LAL
  • 04:27Results: Representative Detection of Endotoxin in PEGylated Liposomal Doxorubicin Using LAL Assays
  • 05:12Conclusion

Summary

Automatic Translation

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Påvisning af endotoksiner i industrielt fremstillet nanomateriale repræsenterer en af de store udfordringer inden for Nanomedicin. Vi præsenterer her, et casestudie, der beskriver rammerne består af tre forskellige LAL formater til at vurdere potentielle endotoxin forurening i nanopartikler.

Tags

EndotoxinNano-formulationsLimulus Amoebocyte Lysate (LAL) AssaysCalibration StandardControl Standard EndotoxinLAL Grade WaterQuality ControlInstrument SettingsTest IDData GroupHardwareLAL MethodSerial NumberSystem IDSerial PortSample IDNegative ControlStandard CurveTest SamplesInhibition Enhancement ControlDilution

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