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Påvisning af Endotoxin i Nano-formuleringer bruger Limulus Amoebocyte Lysate (LAL) Assays
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays
DOI:

06:15 min

January 30, 2019

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Chapters

  • 00:04Title
  • 00:32Turbidity Limulus Amoebocyte Lysate (LAL) Assay
  • 02:26Sample Loading and Preparation and Gel-Clot LAL
  • 04:27Results: Representative Detection of Endotoxin in PEGylated Liposomal Doxorubicin Using LAL Assays
  • 05:12Conclusion

Summary

Automatic Translation

Påvisning af endotoksiner i industrielt fremstillet nanomateriale repræsenterer en af de store udfordringer inden for Nanomedicin. Vi præsenterer her, et casestudie, der beskriver rammerne består af tre forskellige LAL formater til at vurdere potentielle endotoxin forurening i nanopartikler.

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