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Journal / Developmental Biology / Strømlinjeformet 3D lillehjernen differensiering…
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Developmental Biology

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JoVE Journal Developmental Biology
Strømlinjeformet 3D lillehjernen differensiering protokoll med valgfri 2D modifikasjon
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Strømlinjeformet 3D lillehjernen differensiering protokoll med valgfri 2D modifikasjon

Article DOI: 10.3791/56888-v • 10:15 min • December 9th, 2017
December 9th, 2017 •
1, 1,2
1Department of Pediatrics/Child Neurology, Amsterdam Neuroscience, VU University Medical Center, 2Department of Complex Trait Genetics, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam

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Vi beskriver en forenklet 3D differensiering protokoll for hPSCs, bruker definert medium og redusert vekst faktorer, generere celle aggregat med tidlig neuroepithelial strukturer og positivt for lillehjernen-assosiert markører, samt en valgfri 2D modifisering for å skille celler som en monolayer til å generere functional nerveceller.

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Utviklingspsykologi biologi problemet 130 3D organoid pluripotent stamceller lillehjernen granule celle cortex
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