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Journal / Developmental Biology / Generere 3D-sfærer og 2D luft-væske grensesnitt…
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Developmental Biology

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JoVE Journal Developmental Biology
Generere 3D-sfærer og 2D luft-væske grensesnitt kulturer av menneskelig indusert pluripotent stamcelle-avledet type 2 Alveolar epitelceller
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Generere 3D-sfærer og 2D luft-væske grensesnitt kulturer av menneskelig indusert pluripotent stamcelle-avledet type 2 Alveolar epitelceller

Article DOI: 10.3791/63875-v • 07:02 min • April 15th, 2022
April 15th, 2022 •
*1,2,3, *1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, *1,2, *1,2
1Center for Regenerative Medicine, Boston University and Boston Medical Center, 2The Pulmonary Center and Department of Medicine, Boston University School of Medicine, 3QIMR Berghofer Medical Research Institute
* These authors contributed equally

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Den nåværende protokollen beskriver menneskeskapte pluripotente stamcelleavledede type 2 alveolar epitellignende celler (iAT2s). Disse cellene kan dyrkes som selvfornyende sfærer i 3D-kultur eller tilpasses luft-væske grensesnitt (ALI) kultur.

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Utviklingsbiologi utgave 182
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