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JoVE Journal Behavior
En gravende/Tunneling Assay til påvisning af hypoxi i Drosophila melanogaster larver
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En gravende/Tunneling Assay til påvisning af hypoxi i Drosophila melanogaster larver

Article DOI: 10.3791/57131-v • 09:04 min • March 27th, 2018
March 27th, 2018 •
1,2, 1, 1
1Department of Biosciences, Rice University, 2Yale Medical School

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Protokollen beskriver en simpel analyse for at identificere Drosophila melanogaster larver, der oplever hypoxi under normale atmosfæriske ilt niveauer. Denne protokol tillader hypoksiske larver kan skelnes fra andre mutanter, der viser overlappende fænotyper som træghed eller langsom vækst.

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Adfærd sag 133 D. melanogaster larve hypoxi mad gravende undergrundens tunneling larve vækst luftrøret larve locomotion stakåndet uninflatable
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