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JoVE Journal Genetics
Tilpasning af hybridisering fange af kromatin-associerede proteiner for Proteomics til pattedyrceller
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Tilpasning af hybridisering fange af kromatin-associerede proteiner for Proteomics til pattedyrceller

Article DOI: 10.3791/57140-v • 09:27 min • June 1st, 2018
June 1st, 2018 •
1,2, 1, 1, 1, 1, 3, 3, 1,2
1Department of Genetics, Texas Biomedical Research Institute, 2Department of Internal Medicine-Molecular Medicine, Wake Forest University School of Medicine, 3Department of Chemistry, University of Wisconsin

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Dette er en metode til at identificere nye DNA-interagere proteiner på specifikke mål loci, afhængige af sekvens-specifikke erobringen af crosslinked kromatin for efterfølgende proteom analyser. Ingen forudgående viden om potentielle-bindende proteiner, og heller ikke celle ændringer er nødvendige. Oprindeligt udviklet til gær, er teknologien nu blevet tilpasset for pattedyrsceller.

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Genetik sag 136 HyCCAPP proteomics DNA-protein interaktioner kromatin massespektrometri NFκB DUSP3 DUSP5
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