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Journal / Neuroscience / Combinando Optogenetics con microRNAs…
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Neuroscience

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JoVE Journal Neuroscience
Combinando Optogenetics con microRNAs artificiales para caracterizar los efectos de Gene caída en la función presináptica en circuitos neuronales intactas
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Combinando Optogenetics con microRNAs artificiales para caracterizar los efectos de Gene caída en la función presináptica en circuitos neuronales intactas

Article DOI: 10.3791/57223-v • 09:17 min • March 14th, 2018
March 14th, 2018 •
1, 1, 1
1Center for Synaptic Neuroscience and Technology, Istituto Italiano di Tecnologia

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Este protocolo proporciona un flujo de trabajo sobre cómo combinar artificial microRNA mediada por ARN de interferencia con optogenetics estimular específicamente botones presinápticos con menor expresión de genes selectivos dentro de circuitos neuronales intactos.

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Neurociencia número 133 Optogenetics channelrhodopsin ultrarrápida ChETA microRNA ARN de interferencia caída virus adeno-asociado recombinante rAAV1/2 presináptica sináptica transmisión plasticidad sináptica extracción de RNA
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