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Journal
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Neuroscience
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光遗传学与人工 microRNAs 相结合对完整神经元回路中基因击倒对突触前功能的影响
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits
Please note that all translations are automatically generated.
Click here for the English version.
光遗传学与人工 microRNAs 相结合对完整神经元回路中基因击倒对突触前功能的影响
DOI:
10.3791/57223-v
•
09:17 min
•
March 14, 2018
•
Agnes Thalhammer
,
Fanny Jaudon
,
Lorenzo A. Cingolani
1
Center for Synaptic Neuroscience and Technology
,
Istituto Italiano di Tecnologia
Chapters
00:04
Title
01:09
Extracting RNA from Primary Cultures to Evaluate microRNA Knockdown Efficiency
03:53
Stimulation of Knocked-down Neurons Using Optogenetics
07:13
Results: Knockdown of P/Q-type Voltage-gated Ca
2+
Channel at CA3 to CA1 Synapses
08:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议提供了一个工作流, 如何将人工 microRNA 介导的 RNA 干扰与光遗传学相结合, 以刺激特定突触前 boutons 在完整的神经元回路中的选择性基因表达减少。
Tags
Optogenetics
Artificial MicroRNA
RNA Interference
Presynaptic Function
Neuronal Circuits
Synaptic Physiology
Presynaptic Proteins
Neuronal Culture
Gene Knockdown
Acute Brain Slices
In Vivo
Corticoculturing
AraC
RNA Extraction
Chloroform
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