Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits

Agnes Thalhammer; Fanny Jaudon; Lorenzo A. Cingolani

doi:10.3791/57223

Journal

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Neuroscience

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光遗传学与人工 microRNAs 相结合对完整神经元回路中基因击倒对突触前功能的影响

Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits

JoVE Journal
Neuroscience

This content is Free Access.

JoVE Journal Neuroscience

Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits

光遗传学与人工 microRNAs 相结合对完整神经元回路中基因击倒对突触前功能的影响

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10,134 Views

•

09:17 min

•

March 14, 2018

DOI:

10.3791/57223-v

DOI:

10.3791/57223-v

•

09:17 min

March 14, 2018

•

3 Views

Agnes Thalhammer¹, Fanny Jaudon¹, Lorenzo A. Cingolani¹

¹Center for Synaptic Neuroscience and Technology,Istituto Italiano di Tecnologia

Transcript

Summary

该协议提供了一个工作流, 如何将人工 microRNA 介导的 RNA 干扰与光遗传学相结合, 以刺激特定突触前 boutons 在完整的神经元回路中的选择性基因表达减少。

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Optogenetics Artificial MicroRNA RNA Interference Presynaptic Function Neuronal Circuits Synaptic Physiology Presynaptic Proteins Neuronal Culture Gene Knockdown Acute Brain Slices In Vivo Corticoculturing AraC RNA Extraction Chloroform

光遗传学与人工 microRNAs 相结合对完整神经元回路中基因击倒对突触前功能的影响

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