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Immunology and Infection

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JoVE Journal Immunology and Infection
Replikering av bestilt, Nonredundant bibliotek av Pseudomonas aeruginosa belastning PA14 Transposon innsetting mutanter
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Replikering av bestilt, Nonredundant bibliotek av Pseudomonas aeruginosa belastning PA14 Transposon innsetting mutanter

Article DOI: 10.3791/57298-v • 11:35 min • May 4th, 2018
May 4th, 2018 •
*1, *1, 2, 1, 1, 2,3, 1,4, 1,4
1Department of Pediatrics, Mucosal Immunology and Biology Research Center, Massachusetts General Hospital, 2Department of Molecular Biology, Massachusetts General Hospital, 3Department of Genetics, Harvard Medical School, 4Department of Pediatrics, Harvard Medical School
* These authors contributed equally

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Pseudomonas aeruginosa infeksjon forårsaker betydelig sykelighet i sårbare verter. Nonredundant transposon innsetting mutant biblioteket av P. aeruginosa belastning PA14, som PA14NR angir, forenkler analyse av genet funksjonalitet i mange prosesser. Presenteres her er en protokoll for å generere høy kvalitet kopier av PA14NR sette mutant biblioteket.

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Immunologi og infeksjon problemet 135 Pseudomonas aeruginosa mariner transposon mutant bibliotek mutagenese mikrobiologi opportunistisk infeksjon immunologi
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