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JoVE Journal Biochemistry
Rensning og rekonstituering af TRPV1 for spektroskopisk analyse
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Rensning og rekonstituering af TRPV1 for spektroskopisk analyse

Article DOI: 10.3791/57796-v • 11:53 min • July 3rd, 2018
July 3rd, 2018 •
1, 2, 1,3, 1, 1
1Department of Physiology, University of Tennessee Health Science Center, 2Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, 3CuriRX, Inc.

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Denne artikel beskriver specifikke metoder til at opnå biokemiske mængder af vaskemiddel-solubilized TRPV1 for spektroskopisk analyse. De kombinerede protokoller giver biokemiske og biofysiske værktøjer, som kan tilpasses til at lette strukturelle og funktionelle studier til pattedyr Ionkanaler i en membran-kontrolleret miljø.

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Biokemi sag 137 forbigående receptor potentielle vanilloid 1 TRPV1 rensning rekonstitution spin-mærkning EPR spektroskopi HJORTE spektroskopi
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