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JoVE Journal Biochemistry
Rekonstitution af Septin-samling ved membraner for at studere biofysiske egenskaber og funktioner
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Rekonstitution af Septin-samling ved membraner for at studere biofysiske egenskaber og funktioner

Article DOI: 10.3791/64090-v • 06:32 min • July 28th, 2022
July 28th, 2022 •
*1, *2, 1,3, 1,2,3
1Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, 2Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, 3Department of Biology, University of North Carolina at Chapel Hill
* These authors contributed equally

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Cellefri rekonstitution har været et vigtigt værktøj til at forstå cytoskeletsamlingen, og arbejdet i det sidste årti har etableret tilgange til at studere septindynamik i minimale systemer. Præsenteret her er tre komplementære metoder til at observere septinsamling i forskellige membrankontekster: plane dobbeltlag, sfæriske understøtninger og stangstøtter.

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Biokemi udgave 185
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